Spectrophotometer absorbance and transmittance relationship

Difference Between Absorbance and Transmittance

spectrophotometer absorbance and transmittance relationship

Use our online tool to convert absorbance to transmittance or transmission to absorbance using the Lambert-Beer's law equation (A = 2 - log10 %T). Our formula Absorbance is measured with a spectrophotometer, which establishes the light. Because there is a mathematical relationship between solute concentration and light absorption/transmittance, we can use spectrophotometry to determine the. The relationship is also not linear. Absorbance is actually log10I0/IT. Relationship between %Transmittance and light path length and concentration.

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spectrophotometer absorbance and transmittance relationship