Spectrophotometer absorbance and transmittance relationship
Difference Between Absorbance and Transmittance
Use our online tool to convert absorbance to transmittance or transmission to absorbance using the Lambert-Beer's law equation (A = 2 - log10 %T). Our formula Absorbance is measured with a spectrophotometer, which establishes the light. Because there is a mathematical relationship between solute concentration and light absorption/transmittance, we can use spectrophotometry to determine the. The relationship is also not linear. Absorbance is actually log10I0/IT. Relationship between %Transmittance and light path length and concentration.
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